Literature DB >> 10098972

A phosphorus-31 nuclear magnetic resonance study on the complex of chicken gizzard myosin subfragment 1 with adenosine diphosphate.

M Tanokura1, Y Suzuki.   

Abstract

The complex of Mg-ADP with chicken gizzard myosin subfragment 1 (S1), obtained by the treatment with Staphylococcus aureus V8 proteinase, was observed with 31P NMR at various temperatures between 0 and 25 degrees C. The signal of S1.ADP complex was observed at -2 to -3 ppm as a rather broad peak. As compared with the results for rabbit skeletal muscle S1.ADP complex (Tanokura M, Ebashi S: J Biochem 113: 19-21, 1993), the signal was assigned to beta-phosphate of ADP in the S1.ADP complex. The signal of the complex was so broad and weak that the dependences on temperature and magnetic field strength were not clear. The observation suggests the tight interaction of S1 with the phosphate moieties of ADP in the complex and the extremely anisotropic distribution of electrons around phosphorus nuclei.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10098972

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  6 in total

Review 1.  The active site of myosin.

Authors:  I Rayment; C Smith; R G Yount
Journal:  Annu Rev Physiol       Date:  1996       Impact factor: 19.318

Review 2.  The structural basis of the myosin ATPase activity.

Authors:  I Rayment
Journal:  J Biol Chem       Date:  1996-07-05       Impact factor: 5.157

3.  Proteolysis of smooth muscle myosin by Staphylococcus aureus protease: preparation of heavy meromyosin and subfragment 1 with intact 20 000-dalton light chains.

Authors:  M Ikebe; D J Hartshorne
Journal:  Biochemistry       Date:  1985-04-23       Impact factor: 3.162

4.  Phosphorus-31 nuclear magnetic resonance evidence for two conformations of myosin subfragment-1.nucleotide complexes.

Authors:  J W Shriver; B D Sykes
Journal:  Biochemistry       Date:  1981-03-31       Impact factor: 3.162

5.  Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus-31 nuclear magnetic resonance.

Authors:  M Tanokura; S Ebashi
Journal:  J Biochem       Date:  1993-01       Impact factor: 3.387

6.  Energetics and kinetics of interconversion of two myosin subfragment-1.adenosine 5'-diphosphate complexes as viewed by phosphorus-31 nuclear magnetic resonance.

Authors:  J W Shriver; B D Sykes
Journal:  Biochemistry       Date:  1981-10-27       Impact factor: 3.162
  6 in total
  2 in total

1.  Interpretation of ³¹P NMR saturation transfer experiments: what you can't see might confuse you. Focus on "Standard magnetic resonance-based measurements of the Pi→ATP rate do not index the rate of oxidative phosphorylation in cardiac and skeletal muscles".

Authors:  R S Balaban; A P Koretsky
Journal:  Am J Physiol Cell Physiol       Date:  2011-04-13       Impact factor: 4.249

2.  Myosin-catalyzed ATP hydrolysis elucidated by 31P NMR kinetic studies and 1H PFG-diffusion measurements.

Authors:  Zhiyan Song; Kari J Parker; Idorenyin Enoh; Hua Zhao; Olarongbe Olubajo
Journal:  Anal Bioanal Chem       Date:  2009-09-16       Impact factor: 4.142
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.