Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2.

Literature DB >> 10097088

The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2.

J M Rodríguez-Frade¹, A J Vila-Coro, A M de Ana, J P Albar, C Martínez-A, M Mellado.

Abstract

Cytokines interact with hematopoietin superfamily receptors and stimulate receptor dimerization. We demonstrate that chemoattractant cytokines (chemokines) also trigger biological responses through receptor dimerization. Functional responses are induced after pairwise crosslinking of chemokine receptors by bivalent agonistic antichemokine receptor mAb, but not by their Fab fragments. Monocyte chemoattractant protein (MCP)-1-triggered receptor dimerization was studied in human embryonic kidney (HEK)-293 cells cotransfected with genes coding for the CCR2b receptor tagged with YSK or Myc sequences. After MCP-1 stimulation, immunoprecipitation with Myc-specific antibodies revealed YSK-tagged receptors in immunoblotting. Receptor dimerization also was validated by chemical crosslinking in both HEK-293 cells and the human monocytic cell line Mono Mac 1. Finally, we constructed a loss-of-function CCR2bY139F mutant that acted as a dominant negative, blocking signaling through the CCR2 wild-type receptor. This study provides functional support for a model in which the MCP-1 receptor is activated by ligand-induced homodimerization, allowing discussion of the similarities between bacterial and leukocyte chemotaxis.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 1999 PMID： 10097088 PMCID： PMC22345 DOI： 10.1073/pnas.96.7.3628

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

34 in total

Review 1. Chemokines: progress toward identifying molecular targets for therapeutic agents.

Authors: O M Howard; A Ben-Baruch; J J Oppenheim
Journal: Trends Biotechnol Date: 1996-02 Impact factor: 19.536

2. A peptide derived from a beta2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation.

Authors: T E Hebert; S Moffett; J P Morello; T P Loisel; D G Bichet; C Barret; M Bouvier
Journal: J Biol Chem Date: 1996-07-05 Impact factor: 5.157

Review 3. Chemokines and leukocyte traffic.

Authors: M Baggiolini
Journal: Nature Date: 1998-04-09 Impact factor: 49.962

Review 4. Chemokines and HIV-1 second receptors. Confluence of two fields generates optimism in AIDS research.

Authors: M P D'Souza; V A Harden
Journal: Nat Med Date: 1996-12 Impact factor: 53.440

5. Constitutively active mutants of the alpha 1B-adrenergic receptor: role of highly conserved polar amino acids in receptor activation.

Authors: A Scheer; F Fanelli; T Costa; P G De Benedetti; S Cotecchia
Journal: EMBO J Date: 1996-07-15 Impact factor: 11.598

6. Two EGF molecules contribute additively to stabilization of the EGFR dimer.

Authors: M A Lemmon; Z Bu; J E Ladbury; M Zhou; D Pinchasi; I Lax; D M Engelman; J Schlessinger
Journal: EMBO J Date: 1997-01-15 Impact factor: 11.598

7. A comparison of post-receptor signal transduction events in Jurkat cells transfected with either IL-8R1 or IL-8R2. Chemokine mediated activation of p42/p44 MAP-kinase (ERK-2).

Authors: S A Jones; B Moser; M Thelen
Journal: FEBS Lett Date: 1995-05-08 Impact factor: 4.124

8. Cyclic AMP-increasing agents interfere with chemoattractant-induced respiratory burst in neutrophils as a result of the inhibition of phosphatidylinositol 3-kinase rather than receptor-operated Ca2+ influx.

Authors: M U Ahmed; K Hazeki; O Hazeki; T Katada; M Ui
Journal: J Biol Chem Date: 1995-10-06 Impact factor: 5.157

9. RANTES and monocyte chemoattractant protein-1 (MCP-1) play an important role in the inflammatory phase of crescentic nephritis, but only MCP-1 is involved in crescent formation and interstitial fibrosis.

Authors: C M Lloyd; A W Minto; M E Dorf; A Proudfoot; T N Wells; D J Salant; J C Gutierrez-Ramos
Journal: J Exp Med Date: 1997-04-07 Impact factor: 14.307

10. RANTES induces tyrosine kinase activity of stably complexed p125FAK and ZAP-70 in human T cells.

Authors: K B Bacon; M C Szabo; H Yssel; J B Bolen; T J Schall
Journal: J Exp Med Date: 1996-09-01 Impact factor: 14.307

47 in total

1. CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells.

Authors: I I Singer; S Scott; D W Kawka; J Chin; B L Daugherty; J A DeMartino; J DiSalvo; S L Gould; J E Lineberger; L Malkowitz; M D Miller; L Mitnaul; S J Siciliano; M J Staruch; H R Williams; H J Zweerink; M S Springer
Journal: J Virol Date: 2001-04 Impact factor: 5.103

2. Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells.

Authors: Ramesh C Patel; Ujendra Kumar; Don C Lamb; John S Eid; Magalie Rocheville; Michael Grant; Aruna Rani; Theodore Hazlett; Shutish C Patel; Enrico Gratton; Yogesh C Patel
Journal: Proc Natl Acad Sci U S A Date: 2002-03-05 Impact factor: 11.205

Review 3. Chemokine receptors and neural function.

Authors: Charlene Cho; Richard J Miller
Journal: J Neurovirol Date: 2002-12 Impact factor: 2.643

4. Chemokine receptor homo- or heterodimerization activates distinct signaling pathways.

Authors: M Mellado; J M Rodríguez-Frade; A J Vila-Coro; S Fernández; A Martín de Ana; D R Jones; J L Torán; C Martínez-A
Journal: EMBO J Date: 2001-05-15 Impact factor: 11.598

Review 5. Roles of G-protein-coupled receptor dimerization.

Authors: Sonia Terrillon; Michel Bouvier
Journal: EMBO Rep Date: 2004-01 Impact factor: 8.807

6. Blocking HIV-1 infection via CCR5 and CXCR4 receptors by acting in trans on the CCR2 chemokine receptor.

Authors: José Miguel Rodríguez-Frade; Gustavo del Real; Antonio Serrano; Patricia Hernanz-Falcón; Silvia F Soriano; Antonio J Vila-Coro; Ana Martín de Ana; Pilar Lucas; Ignacio Prieto; Carlos Martínez-A; Mario Mellado
Journal: EMBO J Date: 2003-12-11 Impact factor: 11.598