Literature DB >> 10095067

Cloning, expression, and purification of Bacillus stearothermophilus DNA primase and crystallization of the zinc-binding domain.

H Pan1, L E Bird, D B Wigley.   

Abstract

The dnaG gene encoding DNA primase has been isolated from chromosomal DNA of Bacillus stearothermophilus and its entire nucleotide sequence determined. The deduced amino acid sequence comprised 597 amino acid residues and the molecular mass was calculated to be 67068 Da. B. stearothermophilus primase was overexpressed in Escherichia coli and purified to homogeneity. The N-terminal 12 kDa zinc-binding domain has been crystallized. The crystals are of the monoclinic space group P21 with cell dimensions a=36 A, b=59 A, c=46 A, beta=91.8 degrees and diffract to 1.7 A resolution.

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Year:  1999        PMID： 10095067     DOI： 10.1016/s0167-4781(99)00025-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Class-specific restrictions define primase interactions with DNA template and replicative helicase.

Authors:  Marilynn A Larson; Mark A Griep; Rafael Bressani; Kiran Chintakayala; Panos Soultanas; Steven H Hinrichs
Journal:  Nucleic Acids Res       Date:  2010-06-30       Impact factor: 16.971

2.  Conserved residues of the C-terminal p16 domain of primase are involved in modulating the activity of the bacterial primosome.

Authors:  Kiran Chintakayala; Marilynn A Larson; Mark A Griep; Steven H Hinrichs; Panos Soultanas
Journal:  Mol Microbiol       Date:  2008-04       Impact factor: 3.501

3.  Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro.

Authors:  Kiran Chintakayala; Cristina Machón; Anna Haroniti; Marilyn A Larson; Steven H Hinrichs; Mark A Griep; Panos Soultanas
Journal:  Mol Microbiol       Date:  2009-04       Impact factor: 3.501

4.  Mapping protein-protein interactions within a stable complex of DNA primase and DnaB helicase from Bacillus stearothermophilus.

Authors:  L E Bird; H Pan; P Soultanas; D B Wigley
Journal:  Biochemistry       Date:  2000-01-11       Impact factor: 3.162

5.  Structural Insight into the Specific DNA Template Binding to DnaG primase in Bacteria.

Authors:  Yingqin Zhou; Hao Luo; Zhongchuan Liu; Mu Yang; Xiaoyun Pang; Fei Sun; Ganggang Wang
Journal:  Sci Rep       Date:  2017-04-06       Impact factor: 4.379

6.  A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus.

Authors:  P Soultanas
Journal:  Nucleic Acids Res       Date:  2002-02-15       Impact factor: 16.971

7.  Domain swapping reveals that the C- and N-terminal domains of DnaG and DnaB, respectively, are functional homologues.

Authors:  Kiran Chintakayala; Marilynn A Larson; William H Grainger; David J Scott; Mark A Griep; Steven H Hinrichs; Panos Soultanas
Journal:  Mol Microbiol       Date:  2007-03       Impact factor: 3.501
  7 in total

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