Literature DB >> 1009171

Purification of tRNATrp, tRNAVal, and partial purification of tRNAIle and tRNAMfet from beef liver.

M Fournier, M Dorizzi, C Sarger, J Labouresse.   

Abstract

tRNATrp from beef lever has been purified by classical chromatographical methods. Total tRNA, prepared on a large scale (total aminoacid acceptance 1280 pmol/A260 unit) was submitted to chromatography on benzoylated-DEAE cellulose, then DEAE Sephadex. The major species accepting tryptophan issued from the second chromatography was aminoacylated with [14C] tryptophan and chromatographed on benzoylated-DEAE cellulose. The tRNA carrying the radioactive label was eluted in the ethanolic region. After stripping, the resulting tRNATrp has an acceptance of 1800 pmol/A260 unit. No isoacceptors could be demonstrated by chromatography of the pure species on RCP 5 in 6 M urea. The yield in pure tRNATrp was currently in the range of 25 to 30 percent of the total tryptophan acceptance of the starting curde tRNA.

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Year:  1976        PMID： 1009171     DOI： 10.1016/s0300-9084(76)80114-9

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  17 in total

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3.  Two step synthesis of (-) strong-stop DNA by avian and murine reverse transcriptases in vitro.

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4.  Reverse transcriptase mediated binding of primer tRNA to the viral genome.

Authors:  A Araya; L Sarih; S Litvak
Journal:  Nucleic Acids Res       Date:  1979-08-24       Impact factor: 16.971

5.  Study of the interactions between avian myeloblastosis virus reverse transcriptase and primer tRNA. Affinity labeling and inactivation of the enzyme by periodate-treated tRNATrp.

Authors:  A Araya; E Hevia; S Litvak
Journal:  Nucleic Acids Res       Date:  1980-09-11       Impact factor: 16.971

6.  2'-O-methyl-5-formylcytidine (f5Cm), a new modified nucleotide at the 'wobble' of two cytoplasmic tRNAs Leu (NAA) from bovine liver.

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7.  Inhibition of the p66/p51 form of human immunodeficiency virus reverse transcriptase by tRNA(Lys).

Authors:  B Bordier; L Tarrago-Litvak; M L Sallafranque-Andreola; D Robert; D Tharaud; M Fournier; P J Barr; S Litvak; L Sarih-Cottin
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

8.  Interactions between avian myeloblastosis reverse transcriptase and tRNATrp. Mapping of complexed tRNA with chemicals and nucleases.

Authors:  M Garret; P Romby; R Giegé; S Litvak
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971

9.  tRNATrp (bovine) binding to the reverse transcriptase of avian myeloblastosis virus and function as a heterologous primer.

Authors:  B M Baroudy; M Fournier; J Labouesse; T S Papas; J G Chirikjian
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

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Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971
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