AIMS/ BACKGROUND: We evaluated the retinal toxicity of spartanamicin B and its efficacy in a rabbit model of Candidal endophthalmitis. METHODS: Toxicity. Fourteen albino rabbit eyes were injected intravitreally with spartanamicin B (1, 2, 5, 10, 25, 50, and 100 microg); 2 eyes received the vehicle alone. Efficacy. Ten rabbit eyes were inoculated with 3000 Candida organisms which caused endophthalmitis in 24 hours. Eight eyes were treated with intravitreal spartanamicin B in doses of 1, 2, 5, or 10 microg/0.1 ml; 2 untreated eyes served as controls. RESULTS: Toxicity. The eyes injected with < or =10 microg and the control group eyes exhibited no clinical, histological, or electroretinographic evidence of retinal toxicity. Doses >10 microg caused vitritis. Efficacy. Clinical examination of the treated eyes showed a gradual improvement over 3-6 days; mild opacities remained until day 14. Results of cultures performed 15 days after infection were negative in all treated eyes. CONCLUSIONS: Intravitreally injected spartanamicin B (10 microg) is nontoxic to the retina, effective against C. albicans in the endophthalmitis model in the rabbit eye and caused no discernible histological changes in the retina.
AIMS/ BACKGROUND: We evaluated the retinal toxicity of spartanamicin B and its efficacy in a rabbit model of Candidal endophthalmitis. METHODS:Toxicity. Fourteen albino rabbit eyes were injected intravitreally with spartanamicin B (1, 2, 5, 10, 25, 50, and 100 microg); 2 eyes received the vehicle alone. Efficacy. Ten rabbit eyes were inoculated with 3000 Candida organisms which caused endophthalmitis in 24 hours. Eight eyes were treated with intravitreal spartanamicin B in doses of 1, 2, 5, or 10 microg/0.1 ml; 2 untreated eyes served as controls. RESULTS:Toxicity. The eyes injected with < or =10 microg and the control group eyes exhibited no clinical, histological, or electroretinographic evidence of retinal toxicity. Doses >10 microg caused vitritis. Efficacy. Clinical examination of the treated eyes showed a gradual improvement over 3-6 days; mild opacities remained until day 14. Results of cultures performed 15 days after infection were negative in all treated eyes. CONCLUSIONS: Intravitreally injected spartanamicin B (10 microg) is nontoxic to the retina, effective against C. albicans in the endophthalmitis model in the rabbit eye and caused no discernible histological changes in the retina.
Authors: Deb K Mojumder; Francis A Concepcion; Shil K Patel; Andrew J Barkmeier; Petros E Carvounis; John H Wilson; Eric R Holz; Theodore G Wensel Journal: Invest Ophthalmol Vis Sci Date: 2010-05-26 Impact factor: 4.799
Authors: Mohamed S Hifnawy; Mohamed M Fouda; Ahmed M Sayed; Rabab Mohammed; Hossam M Hassan; Sameh F AbouZid; Mostafa E Rateb; Alexander Keller; Martina Adamek; Nadine Ziemert; Usama Ramadan Abdelmohsen Journal: RSC Adv Date: 2020-06-08 Impact factor: 4.036
Authors: Hua Gao; Mark Pennesi; Kekul Shah; Xiaoxi Qiao; Seenu M Hariprasad; William F Mieler; Samuel M Wu; Eric R Holz Journal: Trans Am Ophthalmol Soc Date: 2003