Literature DB >> 10089480

Production of crystals of human aldose reductase with very high resolution diffraction.

V Lamour1, P Barth, H Rogniaux, A Poterszman, E Howard, A Mitschler, A Van Dorsselaer, A Podjarny, D Moras.   

Abstract

As the action of human aldose reductase (hAR) is thought to be linked to the pathogenesis of diabetic complications, much effort has been directed towards the analysis of the catalytic mechanism and the development of specific inhibitors. Here, the crystallization of recombinant hAR with its cofactor NADP+ at 277 K in the presence of the precipitating agent PEG 6000 is reported. The crystals diffract to high resolution (1.1 A) and belong to the P21 space group with unit-cell parameters a = 49.97, b = 67.14, c = 48. 02 A, beta = 92.2 degrees with one molecule per asymmetric unit. Seleno-substituted hAR crystals were also produced and diffract to 1. 7 A on a conventional X-ray source.

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Year:  1999        PMID： 10089480     DOI： 10.1107/s0907444998013365

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  6 in total

1.  Accurate protein crystallography at ultra-high resolution: valence electron distribution in crambin.

Authors:  C Jelsch; M M Teeter; V Lamzin; V Pichon-Pesme; R H Blessing; C Lecomte
Journal:  Proc Natl Acad Sci U S A       Date:  2000-03-28       Impact factor: 11.205

2.  Electrostatic fields near the active site of human aldose reductase: 2. New inhibitors and complications caused by hydrogen bonds.

Authors:  Lin Xu; Aina E Cohen; Steven G Boxer
Journal:  Biochemistry       Date:  2011-09-06       Impact factor: 3.162

3.  Crystal packing modifies ligand binding affinity: the case of aldose reductase.

Authors:  Alexandra Cousido-Siah; Tatiana Petrova; Isabelle Hazemann; André Mitschler; Francesc X Ruiz; Eduardo Howard; Stephan Ginell; Cédric Atmanene; Alain Van Dorsselaer; Sarah Sanglier-Cianférani; Andrzej Joachimiak; Alberto Podjarny
Journal:  Proteins       Date:  2012-07-28

4.  Electrostatic complementarity in an aldose reductase complex from ultra-high-resolution crystallography and first-principles calculations.

Authors:  Nicolas Muzet; Benoît Guillot; Christian Jelsch; Eduardo Howard; Claude Lecomte
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-10       Impact factor: 11.205

5.  On the use of logarithmic scales for analysis of diffraction data.

Authors:  Alexandre Urzhumtsev; Pavel V Afonine; Paul D Adams
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-11-17

6.  Which Properties Allow Ligands to Open and Bind to the Transient Binding Pocket of Human Aldose Reductase?

Authors:  Anna Sandner; Khang Ngo; Christoph P Sager; Frithjof Scheer; Michael Daude; Wibke E Diederich; Andreas Heine; Gerhard Klebe
Journal:  Biomolecules       Date:  2021-12-06
  6 in total

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