Literature DB >> 1008863

Phospholipid requirement for dimethylnitrosamine demethylation by hamster hepatic microsomal cytochrome P-450 enzyme system.

P D Lotlikar, W J Baldy, J Nyce, E N Dwyer.   

Abstract

Extraction with butan-1-ol of freeze-dried microsomal fractions from livers of 3-methyl-cholarthrene-pre-treated hamsters removed about 90% of the total lipid content, but the lipid remaining proved sufficient for the cytochrome P-450 enzyme system to retain about 15-40% of its original catalytic activity for dimethylnitrosamine demethylation. Addition of butan-1-ol-extracted total phospholipid or phosphatidylcholine could not restore any activity, whereas the addition of the synthetic phospholipid dilauroyl phosphatidylcholine was able to restore almost complete activity. Synthetic dipalmitoyl or distearoyl phosphatidylcholine was ineffective in restoring the activity in this reconstituted system.

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Year:  1976        PMID： 1008863      PMCID： PMC1164247          DOI： 10.1042/bj1600401

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

2.  THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

Authors:  T OMURA; R SATO
Journal:  J Biol Chem       Date:  1964-07       Impact factor: 5.157

3.  A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS.

Authors:  J C DITTMER; R L LESTER
Journal:  J Lipid Res       Date:  1964-01       Impact factor: 5.922

4.  Phosphorus assay in column chromatography.

Authors:  G R BARTLETT
Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  Immunochemical studies on electron transport chains involving cytochrome P-450. I. Effects of antibodies to pig liver microsomal reduced triphosphopyridine nucleotide-cytochrome c reductase and the non-heme iron protein from bovine adrenocortical mitochondria.

Authors:  B S Masters; J Baron; W E Taylor; E L Isaacson; J LoSpalluto
Journal:  J Biol Chem       Date:  1971-07-10       Impact factor: 5.157

7.  The role of cytochrome P-450 and P-448 in drug and steroid hydroxylations.

Authors:  A Y Lu; W Levin; S West; M Jacobson; D Ryan; R Kuntzman; A H Conney
Journal:  Ann N Y Acad Sci       Date:  1973       Impact factor: 5.691

8.  Organic solvent extraction of liver microsomal lipid. I. The requirement of lipid for 3,4-benzpyrene hydroxylase.

Authors:  M Vore; J G Hamilton; A Y Lu
Journal:  Biochem Biophys Res Commun       Date:  1974-02-27       Impact factor: 3.575

9.  Preparation and properties of partially purified cytochrome P-450 and reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase from rabbit liver microsomes.

Authors:  T A van der Hoeven; M J Coon
Journal:  J Biol Chem       Date:  1974-10-10       Impact factor: 5.157

10.  Partial resolution of the mixed-function oxidase system of bovine adrenocortical microsomes into two fractions in the absence of detergents.

Authors:  S Narasimhulu
Journal:  Drug Metab Dispos       Date:  1974 Nov-Dec       Impact factor: 3.922
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  1 in total

1.  Phospholipid requirement for 2-acetamidofluorene N-and ring-hydroxylation by hamster liver microsomal cytochrome P-450 enzyme system.

Authors:  P D Lotlikar; E N Dwyer; W J Baldy; J Nyce
Journal:  Biochem J       Date:  1977-12-15       Impact factor: 3.857
  1 in total

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