| Literature DB >> 10087618 |
J Ellenberg1, J Lippincott-Schwartz, J F Presley.
Abstract
Green fluorescent protein (GFP) has become an important tool in cell biology and is widely used as a reporter for imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.Mesh：
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Year: 1999 PMID： 10087618 DOI： 10.1016/s0962-8924(98)01420-2
Source DB: PubMed Journal: Trends Cell Biol ISSN： 0962-8924 Impact factor: 20.808