| Literature DB >> 10076907 |
M Green1, D Rogers, P Russell, A J Stagg, D L Bell, S M Eley, R W Titball, E D Williamson.
Abstract
In this study, we have shown that severe combined immunodeficient/beige mice reconstituted with hyperimmune Balb/c lymphocytes can be used as a model to demonstrate adoptive and passive protection against plague infection. Reconstitution of severe combined immunodeficient/beige mice was successful in nine out of ten mice as demonstrated by spleen colonisation and sustained circulating immunoglobulin titres. Furthermore, an increase in antibody titre was evident after a booster immunisation of reconstituted mice. Presence of circulating antibody correlated with protection against a systemic plague challenge and indicated that in reconstituted mice adoptive transfer of a functional immune system had occurred. The severe combined immunodeficient/beige mouse was also used to demonstrate passive protection against inhaled and systemic plague infection. The reconstituted severe combined immunodeficient/beige mouse model demonstrating protective immunity against plague will be further developed to identify the immune cell subsets responsible for this protection.Entities:
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Year: 1999 PMID： 10076907 DOI： 10.1111/j.1574-695X.1999.tb01229.x
Source DB: PubMed Journal: FEMS Immunol Med Microbiol ISSN： 0928-8244