Literature DB >> 10074529

Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep.

R J Whittington1, I Marsh, S McAllister, M J Turner, D J Marshall, C A Fraser.   

Abstract

Definitive diagnosis of Johne's disease in ruminants depends on confirming the presence of the causative bacterium, Mycobacterium avium subsp. paratuberculosis, in tissues of the host. This is readily achieved in most ruminant species by culture. However, culture of clinical specimens from sheep in many countries has been unrewarding. Such a culture from sheep was achieved recently in Australia by using a radiometric culture medium. The aims of the present study were to evaluate the culture of M. avium subsp. paratuberculosis from sheep by using modified BACTEC 12B radiometric medium, to determine the sensitivity of culture in relation to histopathology, and to evaluate a range of solid media. Culture of M. avium subsp. paratuberculosis from sheep with Johne's disease is a sensitive method of diagnosis: intestinal tissues from all 43 animals with multibacillary disease and all 22 animals with paucibacillary disease were culture positive, while 98% of feces from 53 animals with multibacillary disease and 48% of feces from 31 animals with paucibacillary disease were culture positive. Of sheep without histological evidence of Johne's disease from infected flocks, intestinal tissue from 32% of 41 were culture positive, while feces from 17% of 41 were culture positive. Consequently, culture is recommended as the "gold standard" test for detection of ovine Johne's disease. Of the wide range of solid media that were evaluated, only modified Middlebrook 7H10 and 7H11 agars, which were very similar in composition to modified BACTEC 12B medium, yielded growth of ovine strains of M. avium subsp. paratuberculosis. The sensitivity of detection of M. avium subsp. paratuberculosis on solid media was slightly lower than that in modified BACTEC 12B radiometric medium. Both egg yolk and mycobactin J were essential additives for growth of ovine strains of M. avium subsp. paratuberculosis in both liquid and solid media.

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Year:  1999        PMID: 10074529      PMCID: PMC88652     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  23 in total

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Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

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Journal:  Aust Vet J       Date:  1990-02       Impact factor: 1.281

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Journal:  Vet Microbiol       Date:  1991-08-30       Impact factor: 3.293

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  51 in total

1.  Experimental infection model for Johne's disease in sheep.

Authors:  D J Begg; R O'brien; C G Mackintosh; J F T Griffin
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

2.  Growth, Congo Red agar colony morphotypes and antibiotic susceptibility testing of Mycobacterium avium subspecies paratuberculosis.

Authors:  Nicole M Parrish; Chiew G Ko; James D Dick; Paul B Jones; Jay L E Ellingson
Journal:  Clin Med Res       Date:  2004-05

3.  Identification of Mycobacterium avium subspecies paratuberculosis strains isolated from dairy goats and dairy sheep in Ontario, Canada.

Authors:  Cathy A Bauman; Andria Jones-Bitton; Christina Ahlstrom; Lucy Mutharia; Jeroen De Buck; Jocelyn Jansen; David Kelton; Paula Menzies
Journal:  Can J Vet Res       Date:  2017-10       Impact factor: 1.310

4.  Adsorption of Mycobacterium avium subsp. paratuberculosis to soil particles.

Authors:  Navneet K Dhand; Jenny-Ann L M L Toribio; Richard J Whittington
Journal:  Appl Environ Microbiol       Date:  2009-06-26       Impact factor: 4.792

5.  Transabdominal ultrasonographic findings in goats with paratuberculosis.

Authors:  Mohamed Tharwat; Fahd Al-Sobayil; Mahmoud Hashad; Sébastien Buczinski
Journal:  Can Vet J       Date:  2012-10       Impact factor: 1.008

6.  Isolation of Mycobacterium avium subspecies paratuberculosis from non-ruminant wildlife living in the sheds and on the pastures of Greek sheep and goats.

Authors:  M Florou; L Leontides; P Kostoulas; C Billinis; M Sofia; I Kyriazakis; F Lykotrafitis
Journal:  Epidemiol Infect       Date:  2007-06-20       Impact factor: 2.451

7.  Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium.

Authors:  Richard J Whittington; Ann-Michele Whittington; Anna Waldron; Douglas J Begg; Kumi de Silva; Auriol C Purdie; Karren M Plain
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

8.  Factors affecting isolation and identification of Mycobacterium avium subsp. paratuberculosis from fecal and tissue samples in a liquid culture system.

Authors:  Richard J Whittington
Journal:  J Clin Microbiol       Date:  2009-01-14       Impact factor: 5.948

9.  Environmental survival of Mycobacterium avium subsp. paratuberculosis in different climatic zones of eastern Australia.

Authors:  Jeffrey Eppleston; Douglas J Begg; Navneet K Dhand; Bruce Watt; Richard J Whittington
Journal:  Appl Environ Microbiol       Date:  2014-01-24       Impact factor: 4.792

10.  Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants.

Authors:  Karen Stevenson; Julio Alvarez; Douwe Bakker; Franck Biet; Lucia de Juan; Susan Denham; Zoi Dimareli; Karen Dohmann; Gerald F Gerlach; Ian Heron; Marketa Kopecna; Linda May; Ivo Pavlik; J Michael Sharp; Virginie C Thibault; Peter Willemsen; Ruth N Zadoks; Alastair Greig
Journal:  BMC Microbiol       Date:  2009-10-07       Impact factor: 3.605

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