Literature DB >> 10074069

Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli.

W P Elias1, J R Czeczulin, I R Henderson, L R Trabulsi, J P Nataro.   

Abstract

Several virulence-related genes have been described for prototype enteroaggregative Escherichia coli (EAEC) strain 042, which has been shown to cause diarrhea in human volunteers. Among these factors are the enterotoxins Pet and EAST and the fimbrial antigen aggregative adherence fimbria II (AAF/II), all of which are encoded on the 65-MDa virulence plasmid pAA2. Using nucleotide sequence analysis and insertional mutagenesis, we have found that the genes required for the expression of each of these factors, as well as the transcriptional activator of fimbrial expression AggR, map to a distinct cluster on the pAA2 plasmid map. The cluster is 23 kb in length and includes two regions required for expression of the AAF/II fimbria. These fimbrial biogenesis genes feature a unique organization in which the chaperone, subunit, and transcriptional activator lie in one cluster, whereas the second, unlinked cluster comprises a silent chaperone gene, usher, and invasin reminiscent of Dr family fimbrial clusters. This plasmid-borne virulence locus may represent an important set of virulence determinants in EAEC strains.

Entities:  

Mesh:

Substances:

Year:  1999        PMID: 10074069      PMCID: PMC93575     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  47 in total

1.  Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites.

Authors:  J Davison; M Heusterspreute; N Chevalier; V Ha-Thi; F Brunel
Journal:  Gene       Date:  1987       Impact factor: 3.688

2.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

3.  Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.

Authors:  N T Keen; S Tamaki; D Kobayashi; D Trollinger
Journal:  Gene       Date:  1988-10-15       Impact factor: 3.688

4.  In vitro insertional mutagenesis with a selectable DNA fragment.

Authors:  P Prentki; H M Krisch
Journal:  Gene       Date:  1984-09       Impact factor: 3.688

5.  Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans.

Authors:  M M Levine; P Ristaino; G Marley; C Smyth; S Knutton; E Boedeker; R Black; C Young; M L Clements; C Cheney
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

6.  Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells.

Authors:  J P Nataro; J B Kaper; R Robins-Browne; V Prado; P Vial; M M Levine
Journal:  Pediatr Infect Dis J       Date:  1987-09       Impact factor: 2.129

7.  Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae.

Authors:  R K Taylor; C Manoil; J J Mekalanos
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

8.  Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5 plasmid encoding murine toxin and capsular antigen.

Authors:  L E Lindler; G V Plano; V Burland; G F Mayhew; F R Blattner
Journal:  Infect Immun       Date:  1998-12       Impact factor: 3.441

9.  Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.

Authors:  A R Datta; J B Kaper; A M MacQuillan
Journal:  J Bacteriol       Date:  1984-11       Impact factor: 3.490

10.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

View more
  34 in total

1.  Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites.

Authors:  G P Munson; L G Holcomb; J R Scott
Journal:  Infect Immun       Date:  2001-01       Impact factor: 3.441

2.  Analyzing of expression of novel polypeptide complexes consisting of Shiga toxin B subunit and Adherence Fimbriae of Escherichia coli based on in silico modeling.

Authors:  Zeinab Noroozian; Mana Oloomi; Saeid Bouzari
Journal:  J Mol Model       Date:  2012-04-24       Impact factor: 1.810

3.  The fimbriae of enteroaggregative Escherichia coli induce epithelial inflammation in vitro and in a human intestinal xenograft model.

Authors:  Erik J Boll; Carsten Struve; Anja Sander; Zachary Demma; James P Nataro; Beth A McCormick; Karen A Krogfelt
Journal:  J Infect Dis       Date:  2012-06-21       Impact factor: 5.226

Review 4.  Adherence of diarrheagenic Escherichia coli strains to epithelial cells.

Authors:  Alfredo G Torres; Xin Zhou; James B Kaper
Journal:  Infect Immun       Date:  2005-01       Impact factor: 3.441

5.  New adhesin of enteroaggregative Escherichia coli related to the Afa/Dr/AAF family.

Authors:  Nadia Boisen; Carsten Struve; Flemming Scheutz; Karen A Krogfelt; James P Nataro
Journal:  Infect Immun       Date:  2008-04-28       Impact factor: 3.441

6.  Metabolic flux analysis of Escherichia coli creB and arcA mutants reveals shared control of carbon catabolism under microaerobic growth conditions.

Authors:  Pablo I Nikel; Jiangfeng Zhu; Ka-Yiu San; Beatriz S Méndez; George N Bennett
Journal:  J Bacteriol       Date:  2009-06-26       Impact factor: 3.490

7.  Crystallization and initial crystallographic analysis of AafA: the major adhesive subunit of the enteroaggregative Escherichia coli AAF/II pilus.

Authors:  Yi Yang; James A Garnett; Stephen Matthews
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-03-25

8.  A novel dispersin protein in enteroaggregative Escherichia coli.

Authors:  Jalaluddin Sheikh; John R Czeczulin; Susan Harrington; Susan Hicks; Ian R Henderson; Chantal Le Bouguénec; Pierre Gounon; Alan Phillips; James P Nataro
Journal:  J Clin Invest       Date:  2002-11       Impact factor: 14.808

9.  Nitazoxanide inhibits biofilm production and hemagglutination by enteroaggregative Escherichia coli strains by blocking assembly of AafA fimbriae.

Authors:  Eliah R Shamir; Michelle Warthan; Sareena P Brown; James P Nataro; Richard L Guerrant; Paul S Hoffman
Journal:  Antimicrob Agents Chemother       Date:  2010-01-19       Impact factor: 5.191

10.  The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli.

Authors:  Anna M Snelling; Louissa R Macfarlane-Smith; Jonathan N Fletcher; Iruka N Okeke
Journal:  BMC Microbiol       Date:  2009-12-21       Impact factor: 3.605

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.