B Wang1, P Yin, L Kong. 1. Department of Pathology, Henan Provincial Hospital, Zhengzhou.
Abstract
OBJECTIVE: To study the cytogenesis of colorectal carcinoma and to look for colorectal carcinoma related chromosomal fragility sites which could facilitate screening of high risk colorectal carcinoma patients. METHODS: 20 cases of surgically resected colorectal carcinomas and 4 cell lines were analysed cytogenetically. RESULTS: Most of the tumor cells were heteroploid, the chromosome number was predominately hypodiploid. Karyotypic analysis demonstrated an increase of chromosome 13, and loss of chromosome 17 and chromosome 1 appeared frequently. The most frequently found structural abnormalities in colorectal carcinoma were breakpoint 1q21 and 1p13. Highly non-random cancer chromosome breakpoints and fragile sites were compared with the oncogene locus and found that their locus or neighboring locus was 1q21. CONCLUSION: The results suggest that breakpoint 1q21 may be related to tumorgenesis and may be useful in screening and preventing colorectal carcinoma.
OBJECTIVE: To study the cytogenesis of colorectal carcinoma and to look for colorectal carcinoma related chromosomal fragility sites which could facilitate screening of high risk colorectal carcinomapatients. METHODS: 20 cases of surgically resected colorectal carcinomas and 4 cell lines were analysed cytogenetically. RESULTS: Most of the tumor cells were heteroploid, the chromosome number was predominately hypodiploid. Karyotypic analysis demonstrated an increase of chromosome 13, and loss of chromosome 17 and chromosome 1 appeared frequently. The most frequently found structural abnormalities in colorectal carcinoma were breakpoint 1q21 and 1p13. Highly non-random cancer chromosome breakpoints and fragile sites were compared with the oncogene locus and found that their locus or neighboring locus was 1q21. CONCLUSION: The results suggest that breakpoint 1q21 may be related to tumorgenesis and may be useful in screening and preventing colorectal carcinoma.