| Literature DB >> 10070951 |
O N Tucker1, A J Dannenberg, E K Yang, F Zhang, L Teng, J M Daly, R A Soslow, J L Masferrer, B M Woerner, A T Koki, T J Fahey.
Abstract
A large body of evidence suggests that cyclooxygenase-2 (COX-2) is important in gastrointestinal cancer. The purpose of this study was to determine whether COX-2 was expressed in adenocarcinoma of the human pancreas. Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in pancreatic tissue. Levels of COX-2 mRNA were increased by >60-fold in pancreatic cancer compared to adjacent nontumorous tissue. COX-2 protein was present in 9 of 10 cases of adenocarcinoma of the pancreas but was undetectable in nontumorous pancreatic tissue. Immunohistochemical analysis showed that COX-2 was expressed in malignant epithelial cells. In cultured human pancreatic cancer cells, levels of COX-2 mRNA and protein were induced by treatment with tumor-promoting phorbol esters. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of pancreatic cancer.Entities:
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Year: 1999 PMID： 10070951
Source DB: PubMed Journal: Cancer Res ISSN： 0008-5472 Impact factor: 12.701