AMPA/kainate receptors permeable to divalent cations in amphibian central nervous system.

Glutamate receptors have been studied extensively in mammals but less explored in lower vertebrates. These receptors are present in amphibians. Using a recent method based upon agonist-induced cobalt uptake, we were able to detect the presence of functional alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptors permeable to divalent cations in tadpoles and in adults. The uptake specificity was checked by co-application of an antagonist. We studied the distribution of receptor-bearing cells in the principal brain regions. The distribution was similar in the two species studied: Rana esculenta (green frog) and Bufo bufo (common toad). The high number of cobalt-positive cells suggests that the AMPA/kainate receptors permeable to divalent cations play an important role in the anuran nervous system.