| Literature DB >> 10069411 |
Y Miyahira1, S Kobayashi, T Takeuchi, T Kamiyama, T Nara, J Nakajima-Shimada, T Aoki.
Abstract
Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circum-sporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.Entities:
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Year: 1999 PMID： 10069411 DOI： 10.1093/intimm/11.2.133
Source DB: PubMed Journal: Int Immunol ISSN： 0953-8178 Impact factor: 4.823