Detection of antigens in mycelial and in arthroconidial phases of Trichophyton mentagrophytes.

Protein pattern changes were investigated in the filamentous fungus Trichophyton mentagrophytes during the morphological transition induced by increased temperature and higher CO2 partial pressure in cultivation atmosphere. The differences between the mycelial and the arthroconidial phase were characterized by SDS-PAGE and by immunodetection with mouse polyclonal antibodies. The components found by Western blotting in mycelia were 88, 86, 32, 29, 19.5, 18.5 kDa, in arthroconidia 108, 92, 88, 66, 56, 41, 39, 19.5 kDa. The results suggest the participation of some heat shock associated proteins of T. mentagrophytes in host immune response against mycotic infection.