| Literature DB >> 10067277 |
T Ledoan1, R Auger, A Benjahad, J P Tenu.
Abstract
An easy and rapid method for tritium labeling of deprotected oligonucleotides is proposed. The method consists in performing the reaction of commercial 3H-succinimidyl propionate with a terminal amino group of the oligonucleotide in an organic medium. High specific radioactivity labeling can be achieved with minimal radiolysis during long term storage. The synthesis of the nonradioactive congener having an identical structure to the labeled compound is also described.Entities:
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Year: 1999 PMID： 10067277 DOI： 10.1080/15257779908043074
Source DB: PubMed Journal: Nucleosides Nucleotides ISSN： 0732-8311