| Literature DB >> 10066514 |
Abstract
Restriction-enzyme-mediated integration (REMI), a method for generating nonhomologous integration of transforming DNA into the chromosomes of eukaryotic cells, has been used for insertion mutagenesis and other genetic studies in diverse organisms. Insertion mutations generated by REMI have facilitated the genetic dissection of developmental pathways in Dictyostelium discoidium and the isolation of virulence factors in several plant pathogenic fungi. Recent work indicates that REMI occurs by nonhomologous end joining.Mesh:
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Year: 1998 PMID: 10066514 DOI: 10.1016/s1369-5274(98)80055-6
Source DB: PubMed Journal: Curr Opin Microbiol ISSN: 1369-5274 Impact factor: 7.934