| Literature DB >> 10065736 |
B Kotlan1, N Gruel, B Zafrani, G Füredi, J Foldi, G G Petranyi, W H Fridman, J L Teillaud.
Abstract
Breast medullary carcinoma are heavily infiltrated by B-lymphocytes and associated with a good prognosis despite their high histological grade. We investigated the Ig repertoire of B-lymphocytes infiltrating one such tumour. A single cell suspension was obtained from a tumor specimen by enzymatic digestion. VH, Vkappa, and Vlambda regions were amplified by RT-PCR using mixtures of primers optimized to maximize the diversity of the PCR products. They were then cloned and sequenced. Analysis of 9 VH, 5 Vkappa, and 10 Vlambda sequences using the Kabat database indicated that several VH and VL region subgroups (I, II and III) are expressed by B-lymphocytes infiltrating this tumor. The analysis of CDR3 regions also showed a variability, although some VH and VL clones exhibited identical or nearly identical sequences. Thus, the B-cell infiltration observed in this breast medullary carcinoma does not reflect a monoclonal proliferation and represents an oligoclonal or a polyclonal B-cell proliferation.Entities:
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Year: 1999 PMID： 10065736 DOI： 10.1016/s0165-2478(98)00098-4
Source DB: PubMed Journal: Immunol Lett ISSN： 0165-2478 Impact factor: 3.685