| Literature DB >> 10051847 |
E Kai1, S Sawata, K Ikebukuro, T Iida, T Honda, I Karube.
Abstract
Polymerase chain reaction (PCR) products were detected using a flow injection-type sensor based on surface plasmon resonance. Asymmetric PCR was used to amplify the target DNA sequence, and two products with different length were produced. The novelty of our DNA detection system was that our target DNA was double stranded but the probe binding site, located in the 3'-terminus, was single stranded. This avoids the formation of intra- and intermolecular complexes. This novel design permitted us not only to detect PCR product but also to develop a rapid detection system for the detection of the verotoxin 2 gene of Escherichia coli O157:H7.Entities:
Mesh：
Substances：
Year: 1999 PMID： 10051847 DOI： 10.1021/ac9807161
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986