Literature DB >> 10037811

Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents.

H K Nguyen1, O Fournier, U Asseline, D Dupret, N T Thuong.   

Abstract

The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.

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Year:  1999        PMID： 10037811      PMCID： PMC148343          DOI： 10.1093/nar/27.6.1492

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  11 in total

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Journal:  J Med Genet       Date:  1999-10       Impact factor: 6.318

2.  A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays.

Authors:  T Pastinen; M Raitio; K Lindroos; P Tainola; L Peltonen; A C Syvänen
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3.  Thermodynamic treatment of oligonucleotide duplex-simplex equilibria.

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Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-01       Impact factor: 11.205

4.  Application of DNA array technology for diagnostic microbiology.

Authors:  S A Booth; M A Drebot; G A Tipples; L K Ng
Journal:  Can J Infect Dis       Date:  2000-11

5.  Parallel multiplex thermodynamic analysis of coaxial base stacking in DNA duplexes by oligodeoxyribonucleotide microchips.

Authors:  V A Vasiliskov; D V Prokopenko; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  2001-06-01       Impact factor: 16.971

6.  Detection of mitochondrial single nucleotide polymorphisms using a primer elongation reaction on oligonucleotide microarrays.

Authors:  F Erdogan; R Kirchner; W Mann; H H Ropers; U A Nuber
Journal:  Nucleic Acids Res       Date:  2001-04-01       Impact factor: 16.971

7.  A strongly pairing fifth base: oligonucleotides with a C-nucleoside replacing thymidine.

Authors:  Tanja J Walter; Clemens Richert
Journal:  Nucleic Acids Res       Date:  2018-09-19       Impact factor: 16.971

8.  Clamping down on weak terminal base pairs: oligonucleotides with molecular caps as fidelity-enhancing elements at the 5'- and 3'-terminal residues.

Authors:  Sukunath Narayanan; Julia Gall; Clemens Richert
Journal:  Nucleic Acids Res       Date:  2004-05-20       Impact factor: 16.971

9.  Selectivity of Enzymatic Conversion of Oligonucleotide Probes during Nucleotide Polymorphism Analysis of DNA.

Authors:  O A Vinogradova; D V Pyshnyi
Journal:  Acta Naturae       Date:  2010-04       Impact factor: 1.845

10.  Enzymatic synthesis of structure-free DNA with pseudo-complementary properties.

Authors:  Georges Lahoud; Victor Timoshchuk; Alexandre Lebedev; Miguel de Vega; Margarita Salas; Khalil Arar; Ya-Ming Hou; Howard Gamper
Journal:  Nucleic Acids Res       Date:  2008-04-29       Impact factor: 16.971
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