Literature DB >> 10026155

Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1-4Xyl.

H Wakabayashi1, S Natsuka, T Mega, N Otsuki, M Isaji, M Naotsuka, S Koyama, T Kanamori, K Sakai, S Hase.   

Abstract

O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+ ++-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.

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Year:  1999        PMID： 10026155     DOI： 10.1074/jbc.274.9.5436

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  9 in total

Review 1.  Regulated expression and neural functions of human natural killer-1 (HNK-1) carbohydrate.

Authors:  Yasuhiko Kizuka; Shogo Oka
Journal:  Cell Mol Life Sci       Date:  2012-06-06       Impact factor: 9.261

2.  Involvement of human natural killer-1 (HNK-1) sulfotransferase in the biosynthesis of the GlcUA(3-O-sulfate)-Gal-Gal-Xyl tetrasaccharide found in α-thrombomodulin from human urine.

Authors:  Taishi Hashiguchi; Shuji Mizumoto; Yuko Nishimura; Jun-ichi Tamura; Shuhei Yamada; Kazuyuki Sugahara
Journal:  J Biol Chem       Date:  2011-08-02       Impact factor: 5.157

3.  Recombinant human lecithin-cholesterol acyltransferase Fc fusion: analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region.

Authors:  Chris Spahr; Justin J Kim; Sihong Deng; Paul Kodama; Zhen Xia; Jay Tang; Richard Zhang; Sophia Siu; Noi Nuanmanee; Bram Estes; Jennitte Stevens; Mingyue Zhou; Hsieng S Lu
Journal:  Protein Sci       Date:  2013-12       Impact factor: 6.725

4.  O-glycosylation of glycine-serine linkers in recombinant Fc-fusion proteins: attachment of glycosaminoglycans and other intermediates with phosphorylation at the xylose sugar subunit.

Authors:  Chris Spahr; Stone D-H Shi; Hsieng S Lu
Journal:  MAbs       Date:  2014 Jul-Aug       Impact factor: 5.857

5.  Glycosaminoglycans in Human and Bovine Serum: Detection of Twenty-Four Heparan Sulfate and Chondroitin Sulfate Motifs Including a Novel Sialic Acid-modified Chondroitin Sulfate Linkage Hexasaccharide.

Authors:  Hong Lu; Lynda M McDowell; Daniel R Studelska; Lijuan Zhang
Journal:  Glycobiol Insights       Date:  2010-02-09

6.  2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis.

Authors:  Yuko Tone; Lars C Pedersen; Tomoko Yamamoto; Tomomi Izumikawa; Hiroshi Kitagawa; Junko Nishihara; Jun-Ichi Tamura; Masahiko Negishi; Kazuyuki Sugahara
Journal:  J Biol Chem       Date:  2008-04-09       Impact factor: 5.157

7.  Glycosaminoglycans and sialylated glycans sequentially facilitate Merkel cell polyomavirus infectious entry.

Authors:  Rachel M Schowalter; Diana V Pastrana; Christopher B Buck
Journal:  PLoS Pathog       Date:  2011-07-28       Impact factor: 6.823

8.  A Sulfated Glycosaminoglycan Linkage Region is a Novel Type of Human Natural Killer-1 (HNK-1) Epitope Expressed on Aggrecan in Perineuronal Nets.

Authors:  Keiko Yabuno; Jyoji Morise; Yasuhiko Kizuka; Noritaka Hashii; Nana Kawasaki; Satoru Takahashi; Shinji Miyata; Tomomi Izumikawa; Hiroshi Kitagawa; Hiromu Takematsu; Shogo Oka
Journal:  PLoS One       Date:  2015-12-10       Impact factor: 3.240

9.  Characterization of Glycan Structures of Chondroitin Sulfate-Glycopeptides Facilitated by Sodium Ion-Pairing and Positive Mode LC-MS/MS.

Authors:  Jonas Nilsson; Fredrik Noborn; Alejandro Gomez Toledo; Waqas Nasir; Carina Sihlbom; Göran Larson
Journal:  J Am Soc Mass Spectrom       Date:  2016-11-21       Impact factor: 3.109
  9 in total

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