p16 involvement in primary bladder tumors: analysis of deletions and mutations.

Different studies have proposed that genetic alterations leading to inactivation of a tumor suppressor gene on chromosome 9 is an important early event in bladder tumorigenesis. Recent reports have described the p16 gene as the main target. In order to better define its role, we studied 9p21 deletions by microsatellite analysis and its coding sequence. Forty-eight percent of the 44 samples we studied showed LOH surrounding p16. Three of these 44 samples displayed point mutations in p16 and three others were suspected of homozygous deletion. These results suggest that simultaneous loss of both p16 alleles, by point mutation or homozygous deletion, seems to be infrequent in bladder tumors.