Determination of trichothecenes in cereals.

An effective method for the determination of seven trichothecenes-deoxynivalenol (DON), nivalenol (NIV), T-2 tetraol (T-24), fusarenon-X (FUS-X), diacetoxyscirpenol (DAS), T-2 toxin (T-2), HT-2 toxin (HT-2) in cereals is presented. Gel permeation chromatography on Bio-Beads S-X3 was used for clean-up of acetonitrile-methanol extract. GC-ECD was used for identification and quantification of trifluoroacetylated trichothecenes. The limit of quantitation for the method was in the range 40-200 micrograms/kg. Recoveries at a spiking level of 2 mg/kg ranged from 76 to 100%.