Literature DB >> 1000861

Determination of deuterium-labeled phenylalanine and tyrosine in human plasma with high pressure liquid chromatography and mass spectrometry.

F K Trefz, D J Byrd, M E Blaskovics, W Kochen, P Lutz.   

Abstract

A method is presented for the recovery of deuterated phenylalanine and tyrosine from human plasma. Phenylthiohydantoine derivatives are formed (Edman reaction) which are separated and isolated by high pressure liquid chromatography. The relative concentration of the deuterated amino acid is determined by mass spectrometry. The results obtained from a healthy person after oral loading with 40% monodeuterated L-phenylalanine are presented. The method appears to be suitable for in vivo studies of phenylalanine metabolism in humans.

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Year:  1976        PMID： 1000861     DOI： 10.1016/0009-8981(76)90144-3

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  5 in total

1.  In vivo residual activities of the phenylalanine hydroxylating system in phenylketonuria and variants.

Authors:  F K Trefz; K Bartholomé; H Bickel; P Lutz; H Schmidt; H W Seyberth
Journal:  J Inherit Metab Dis       Date:  1981       Impact factor: 4.982

2.  Significance of the in vivo deuterated phenylalanine load for long-term phenylalanine tolerance and psycho-intellectual outcome in patients with PKU.

Authors:  F K Trefz; U Batzler; T König; U Michel; E Schmidt; H Schmidt; H Bickel
Journal:  Eur J Pediatr       Date:  1990       Impact factor: 3.183

3.  Significant phenylalanine hydroxylation in vivo in patients with classical phenylketonuria.

Authors:  G N Thompson; D Halliday
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808

4.  The contribution of phenylalanine to tyrosine metabolism in vivo. Studies in the post-absorptive and phenylalanine-loaded rat.

Authors:  L L Moldawer; I Kawamura; B R Bistrian; G L Blackburn
Journal:  Biochem J       Date:  1983-03-15       Impact factor: 3.857

5.  Standardized loading test with protein for the differentiation of phenylketonuria from hyperphenylalaninaemia.

Authors:  P Lutz; H Schmidt; G Frey; H Bickel
Journal:  J Inherit Metab Dis       Date:  1982       Impact factor: 4.982
  5 in total

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